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Use of Medicare services related to diabetes care: the impact of rural isolation. Recipient mice were sensitized by footpad injections of dendritic cells (DC) obtained from donor lymph nodes up to 3 days following skin painting, when DC expressed high levels of antigen. The belief that existing screening tools are inaccurate in HIV-infected individuals and the need to refer patients to separate clinics for tuberculosis screening also emerged as barriers. Hybridoma cells were obtained by fusing mouse myeloma cells with spleen cells from the immunized animal.